解剖学报

›› 2009, Vol. 40 ›› Issue (3): 379-384.doi: 10.3969/j.issn.0529-1356.2009.03.007

• 论著 • 上一篇    下一篇

β淀粉样蛋白25～35急性给药和慢性孵育对神经元Ca2+非依赖性的K+电流作用的比较

张鹏俊1 ;杨小荣1; 董斌1;孙萍1 ; 王永2 ; 张策1*    

  1. 1. 山西医科大学生理学教研室，太原 030001; 2. 山西医科大学第二医院神经内科，太原 030001
  • 收稿日期:2009-01-16 修回日期:2009-02-23 出版日期:2009-06-06
  • 通讯作者: 张策

Comparison of the effects of acute administration and chronic exposure of βamyloidpeptide 25-35 on the Ca SUP>2+/SUP>-independent KSUP>+/SUP> currents in rat  BR>hippocampal and cerebral cortical neurons BR>

  1. 1. Department of Physiology, Shanxi Medical University, Taiyuan 030001, China;2. Department of Neurology, the Second Hospital of Shanxi Medical University, Taiyuan 030001, China
  • Received:2009-01-16 Revised:2009-02-23 Online:2009-06-06
  • Contact: ZHANG Ce

关键词: β-淀粉样肽25～35, 电压激活的钾通道, 离子电流, 神经元, 全细胞膜片钳技术, 大鼠

Abstract: P> Objective To study the different effects between the acute administration and chronic exposure of βamyloidpeptide 2535(Aβ25-35) on the Ca2+independent K+ currents in rat hippocampal and cerebral cortical neurons. Methods After different applications (acute administration and chronic exposure) of Aβ25-35 in rat hippocampal or cerebral cortical neurons, the Ca2+independent K+ currents were recorded by wholecell patch clamp technique and the cell viability was detected by Calcein-AM staining. BR> Results The amplitudes of the Ca SUP>2+/SUP>independent K+ currents were significantly decreased by acute administration to AβSUB>25-35/SUB> in acute isolation of hippocampal neurons (EM>n/EM>=11) while the amplitudes were marke

Key words: β-amyloid 25-35, Voltage-gated potassium channel, Current, Neuron, Whole cell patch clamp techniques, Rat

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